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为了探明冷激诱导高温胁迫下番茄幼苗矮化的机理,育苗期间,每天8:00对幼苗分别进行5、10、15℃持续时间依次为10、20、30 min的冷激处理,测试了不同冷激强度下番茄幼苗乙烯释放速率,研究了冷激处理T10℃D10 min(10℃持续10 min)结合不同生长调节物质对番茄幼苗乙烯释放速率、赤霉素(GA3)含量和幼苗生长特性的影响.结果表明:冷激处理刺激了番茄幼苗乙烯的产生,随着冷激温度的降低和冷激时间的延长,冷激诱导乙烯释放的效应显著增强.5℃持续30 min的冷激处理番茄幼苗乙烯产生速率最大,达到60.3 n L·h-1·g-1,为对照的6.5倍;乙烯利(ETH)、硫代硫酸银(STS)、GA3和多效唑(PP333)均不能完全阻止冷激处理T10℃D10 min诱发的高乙烯产生率.冷激处理T10℃D10 min番茄幼苗茎叶GA3含量为80.8μg·g-1,与对照(130.6μg·g-1)相比降低了38.1%.喷施ETH、STS对冷激诱发的幼苗矮化效应无显著影响,而GA3显著减弱了冷激的矮化效应,PP333显著增强了冷激的矮化效应.以株高作为衡量指标,浓度为4.0 mg·L-1的PP333处理,相当于10℃冷激处理.冷激诱导的番茄幼苗矮化效应主要原因在于冷激降低了番茄幼苗茎叶GA3的含量.T10℃D10 min可以在降低幼苗株高的同时不降低幼苗干物质的积累.
In order to explore the mechanism of cold-induced tomato seedling dwarfing under high temperature stress, during the seedling raising period, the seedlings were subjected to cold shock treatment at 5, 10 and 15 ℃ for 10, 20 and 30 min, respectively, The effect of cold shock on the ethylene release rate of tomato seedlings was studied. Effects of cold shock treatment on the ethylene release rate, gibberellic acid (GA3) content and seedling growth characteristics of tomato seedlings with T10 ℃ D10 min (10 ℃ for 10 min) combined with different growth regulators The results showed that cold stimulation stimulated the ethylene production in tomato seedlings and the effect of cold shock on the ethylene release was enhanced significantly with the decrease of cold shock temperature and the prolongation of chilling time.After 30 minutes of cold shock treatment at 5 ℃, The ethylene production rate of tomato seedlings reached the maximum of 60.3 n L · h-1 · g-1, which was 6.5 times that of the control. ETH, STS, GA3 and PP333 could not completely prevent Cold shock treatment of high ethylene production rate induced by T10 ℃ D10 min cold shock treatment T10 ℃ D10 min GA3 content of tomato seedling stems and leaves 80.8μg · g-1, compared with the control (130.6μg · g-1) compared to a decrease of 38.1 % Spraying ETH, STS had no significant effect on the dwarfing effect induced by cold shock GA3 significantly weakened the dwarfing effect of cold shock and PP333 significantly enhanced the dwarfing effect of cold shock.The concentration of PP333 with a concentration of 4.0 mg · L-1 was the equivalent of 10 ℃ cold-shock treatment with plant height as a measure. The main reason for the induced dwarfing effect on tomato seedlings was that cold shock decreased the content of GA3 in the shoots and leaves of tomato seedlings, and T10 ℃ and D10min could reduce the seedling height without reducing the dry matter accumulation.