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目的:比较经不同固定液处理的小鼠肝脏石蜡切片H E染色效果,优化实验方法。方法用3种不同固定液(10%中性福尔马林、4%多聚甲醛、A F固定液)处理小鼠肝脏组织,经常规脱水、透明、浸蜡、包埋、切片,H E染色,观察比较染色效果。结果10%福尔马林、4%多聚甲醛固定的肝组织结构完整、清晰,细胞界限清楚,染色鲜艳;A F固定液固定的肝组织结构不完整,界限不清,胞浆中出现大量空泡。结论10%中性福尔马林和4%多聚甲醛是制作小鼠肝脏石蜡切片HE染色标本的理想固定液。“,”Purpose: To compare of mouse liver paraffin section and HE staining effect treatment with different fixative, optimization of experimental method. Methods: Mouse livers were immersed in three different fixatives (10% buffered formalin, 4% polyformaldehyde, and AF fluid) respectively. The tissue were dehydrated, transparentized and immersed in paraffin as routine procedure. The paraffin embedded liver tissue were sectioned and stained with Hematoxylin and Eosin, liver sections were compared under the light microscope. Results: The tissues fixed with 10% buffered formalin and 4% polyformaldehyde showed complete and clear structure, and the hepatocytes wel stained. The structure of liver tissues fixed with AF fluid was not complete, il-defined, and with large vacuoles in cytoplasm.Conclusion: 10% buffered formalin and 4% polyformaldehyde fixative is the most effective fixative for mouse liver HE staining.