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本文建立了火焰原子吸收法间接测定食物中植酸含量的方法，对测试条件进行了系统的探讨。该方法的线性范围为０～２０μｇ植酸／ｍｌ，相关系数为０．９９９６，检出限为０．１４４μｇ植酸／ｍｌ，相对标准偏差为１．１％～６．６％，１５种样品的加标回收率为９６．１％～１０６．１％，该法与消化测磷法的测定结果比较，差异无显著性。该法灵敏度高，结果准确可靠，操作简便再稀释成浓度为１０μｇＰ／ｍｌ的应用液。１．２．８植酸标准溶液：精确称取０．６６４１ｇ植酸钾（Ｓｉｇｍａ公司，含量为９５．５５％），用水溶解并定容至１００ｍｌ，其浓度为６ｍｇ植酸／ｍｌ。用时再用水逐级稀释成浓度为３００μｇ植酸／ｍｌ的应用液。１．３分析方法１．３．１原子吸收分光光度计参数分析波长２４８．２ｎｍ，狭缝宽度０．２ｎｍ，灯电流５ｍＡ，空气流速１３．５Ｌ／ｍｉｎ，Ｃ２Ｈ２流速２．２Ｌ／ｍｉｎ，测量方式为面积积分。１．３．２标准曲线的绘制分别取植酸标准应用液（３００μｇ植酸／ｍｌ）０．００、２．００、４．００、６．００、８．００、１０．００ｍｌ于２５ｍｌ玻璃离心管中，各加入３．００ｍｌ铁标准溶液和２．００ｍｌ３．５ｍｏｌ／Ｌ氯化钠溶液，均加水至１５．００ｍｌ，?
In this paper, a method of indirect determination of phytic acid in food by flame atomic absorption spectrometry was established, and the test conditions were systematically discussed. The linear range of this method is 0 ~ 20μg phytic acid / ml, the correlation coefficient is 0.9996, the detection limit is 0.144μg phytic acid / ml, the relative standard deviation is 1.1% ~ 6.6%, 15 kinds of samples The spiked recoveries ranged from 96.1% to 106.1%. There was no significant difference between the method and the measured results of digestion and phosphorus determination. The method of high sensitivity, the results are accurate and reliable, easy to use and then diluted to a concentration of 10μgP / ml of the application solution. 1.2.8 Phytic acid standard solution: Weigh accurately 0.6641g potassium phytate (Sigma, content of 95.55%), dissolved in water and dilute to 100ml, the concentration of 6mg phytic acid / ml. When used again with water gradually diluted to a concentration of 300μg phytic acid / ml of the application solution. 1.3 Analysis Method 1.3.1 Atomic Absorption Spectrophotometer Parameter Analysis Wavelength 248.2nm, slit width 0.2nm, lamp current 5mA, air flow rate 13.5L / min, C2H2 flow rate 2.2L / min, measurement Way for the area points. 1.3.2 Standard Curve Draw phytic acid standard application solution (300μg phytic acid / ml) 0.002.00,4.00,6.00,8.00,10.00 ml in 25ml glass centrifuge Tube, each adding 3.00ml iron standard solution and 2.00ml3.5mol / L sodium chloride solution, both water to 15.00ml ,?