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目的：研究苯并卟啉衍生物单环酸A（BPD）光动力杀伤人鼻咽低分化鳞癌CNE2细胞株的作用机理。方法：在体外培养的CNE2细胞株中加入不同浓度的BPD后，用690nm单色光照射仪照射，照光能量分别为1.2J/cm2、2.4J/cm2、4.8J/cm23组。MTT法检测细胞活性。并收集细胞进行电镜检查。结果：在同一光照能量下，不同浓度BPD对CNE2细胞的杀伤作用随BPD剂量的增大而增强，照光能量分别为1.2J/cm2、2.4J/cm2、4.8J/cm23组，其IC50值分别为1958.6ng/ml、506.38ng/ml和343.0ng/ml。电镜检查：BPD晚光组光照15min，细胞体积已比单纯光照射组缩小，整个细胞密度增加，胞质内所有细胞器呈轻度收缩状态；2h，一些细胞胞质出现明显自溶；8h后能见到2种形式的细胞死亡：坏死和细胞凋亡；24h，主要以坏死细胞为主。结论：在同一光照能量下，对CNE2细胞的杀伤作用随BPD剂量的增大而增强，光动力作用引起的肿瘤细胞死亡存在两种时相及方式，在同一条件下出现两种不同的时相及死亡方式，可能与细胞处在不同生长阶段，代谢程度不一，吸收BPD的含量不同有关。
OBJECTIVE: To study the mechanism of benzoporphyrin derivative monocyclic acid A (BPD) photodynamic kill CNE2 cell line in human nasopharyngeal poorly differentiated squamous cell carcinoma. Methods: CNE2 cells cultured in vitro with different concentrations of BPD after irradiation with 690nm monochromatic light irradiation, light energy were 1.2J / cm2, 2.4J / cm2, 4.8J / cm23 group. MTT assay cell activity. Cells were collected for electron microscopy. Results: Under the same light energy, the killing effect of different concentrations of BPD on CNE2 cells was enhanced with the increase of BPD dose, and the light energy were 1.2J / cm2, 2.4J / cm2 and 4.8J / cm23, 1958.6 ng / ml, 506.38 ng / ml and 343.0 ng / ml. Electron microscopy: BPD night light group light 15min, the cell volume has been reduced compared with simple light irradiation group, the entire cell density increased, all organelles showed a slight contraction of the cytoplasm; 2h, some cells cytoplasm obvious autolysis; 8h after See two forms of cell death: necrosis and apoptosis; 24h, mainly necrotic cells. Conclusion: Under the same light energy, the killing effect on CNE2 cells is enhanced with the increase of BPD dose. There are two phases of tumor cell death induced by photodynamic therapy. Under the same conditions, two different phases And the way of death may be related to the different growth stages of cells, the different degrees of metabolism and the content of absorbing BPD.