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目的:观察疏肝健脾方药对非酒精性脂肪性肝病(NAFLD)大鼠肝组织SREBP-1c mRNA及蛋白表达的影响,并探讨其机制。方法:选雄性SPF级SD大鼠55只,随机分为模型组、正常对照组和3个中药干预组,分别为疏肝组、健脾组及合方组。除正常对照组外,各组采用脂肪乳剂灌胃8 w复制大鼠NAFLD模型,同时各药物干预组给予不同药物干预(10 mL/kg),正常对照组和模型组给予等体积蒸馏水灌胃。8 w后处死动物取肝组织,HE染色观察肝脏病理变化;RT-PCR方法检测肝组织SREBP-1c mRNA的表达;免疫组织化学方法检测肝组织SREBP-1c的蛋白表达水平和分布。结果:模型组大鼠肝组织SREBP-1c mRNA表达水平及SREBP-1c蛋白阳性表达率较正常对照组显著升高(P<0.01);各中药干预组大鼠肝组织SREBP-1c mRNA表达水平及SREBP-1c蛋白阳性表达率较模型组显著降低(P<0.05或P<0.01);疏肝组、健脾组大鼠肝组织SREBP-1c mRNA表达率较合方组显著降低(P<0.01)。病理观察显示疏肝、健脾组大鼠肝组织脂肪变最轻。结论:疏肝健脾方药可能是通过下调SREBP-1c mRNA和蛋白的表达来实现抗NAFLD作用,SREBP-1c可能是疏肝健脾方药抗NAFLD的作用靶点之一。
Objective: To observe the effect of Shuganjianpi Decoction on the expression of SREBP-1c mRNA and protein in non-alcoholic fatty liver disease (NAFLD) rat liver and its mechanism. Methods: Fifty-five male SPF SD rats were randomly divided into model group, normal control group and three Chinese medicine intervention groups, namely Shugan group, Jianpi group and Hefang group. In addition to the normal control group, the rats in each group were treated with fat emulsion for 8 weeks and NAFLD rats were injected with different drugs (10 mL / kg). The normal control group and model group were given the same volume of distilled water. The rats were killed after 8 w of the liver tissue, HE staining was used to observe the pathological changes of the liver. RT-PCR was used to detect the expression of SREBP-1c mRNA in the liver tissues. The protein expression level and distribution of SREBP-1c in the liver tissue were detected by immunohistochemistry. Results: The expression of SREBP-1c mRNA and the expression of SREBP-1c protein in the model group were significantly higher than those in the normal control group (P <0.01). The expression levels of SREBP-1c mRNA and the expression of SREBP- The positive rate of SREBP-1c protein expression was significantly lower in model group than in model group (P <0.05 or P <0.01). The expression of SREBP-1c mRNA in liver tissue of Shugan and Jianpi groups was significantly lower than that in model group (P <0.01) . Pathological observation showed that the hepatic steatosis was the lightest in Shugan and Jianpi groups. Conclusion: Shuganjianpi Decoction can inhibit the expression of SREBP-1c mRNA and protein to achieve anti-NAFLD effect. SREBP-1c may be one of the targets of anti-NAFLD of Shuganjianpi Decoction.