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目的:探讨益骨汤含药血清对新生大鼠成骨细胞增殖及分泌的胰岛样生长因子I(IGF-I)的影响、方法:采用酶消化法获得新生大鼠的成骨细胞,然后以益骨汤含药血清作用于成骨细胞.用Alamar Blue法检测成骨细胞增殖,ELSA法测定培养上清中IGF-I的含量。结果:益骨汤含药血清能促进成骨细胞的增殖,益骨汤高、中、低剂量组分别与空白组比较,差异有统计学意义(P<0.05或0.01)、益骨汤含药血清作用于成骨细胞能使其IGF-1分泌量升高,益骨汤高、中、低剂量组分别与空白组比较,差异有统计学意义(P<0.05或0.01)结论:益骨汤含药血清能够促进成骨细胞的增殖与分化,同时促进成骨细胞分泌IGF-I的作用。
OBJECTIVE: To investigate the effect of Yigu Decoction-containing serum on the proliferation and secretion of islet-like growth factor-I (IGF-I) in neonatal rat osteoblasts. Methods: Osteoblasts of neonatal rats were obtained by enzymatic digestion, The osteoblasts were treated with Yi Gu Tang containing serum, the proliferation of osteoblasts was detected by Alamar Blue method, and the content of IGF-I in the culture supernatant was determined by ELSA method. Results: The YIJU containing serum could promote the proliferation of osteoblasts. The Yisu Decoction high, medium and low dose groups were significantly different from the blank group (P <0.05 or 0.01) The effect of serum on osteoblasts could increase the secretion of IGF-1, the difference was statistically significant (P <0.05 or 0.01) between Yigou decoction group and the blank group, Drug-containing serum can promote the proliferation and differentiation of osteoblasts, and promote the secretion of IGF-I by osteoblasts.