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采用植物MS平板定殖研究法、盆栽试验和土壤酶活性检测技术研究了生防菌B1619在番茄根部的定殖规律,并评估了B1619对番茄根围土壤微生物和酶活性的影响。结果显示,生防菌株B1619在番茄根部定殖能力较强,在MS平板中,定殖量可达108CFU/g根以上。在盆栽试验中,常规土壤中或在接种青枯菌后,番茄根部生防菌的数量都呈现先下降后上升趋势,最后趋于稳定;在第15天时,定殖量达到106CFU/g根。番茄定植后,B1619对根围土壤中真菌和放线菌具有促进作用,而对细菌则表现为先促进后抑制作用。此外,B1619能够提高土壤蔗糖酶活性而抑制土壤脲酶的活性,B1619施用后第15天,蔗糖酶活性达226.3 mg/g土,比对照高88 mg/g土;土壤脲酶活性则与对照组截然相反,呈下降趋势。
The plant colonization of tomato roots was studied by using the method of plant MS plate colonization, pot experiment and soil enzyme activity testing. The effects of B1619 on soil microbial and enzyme activity in tomato rhizosphere soil were evaluated. The results showed that the biocontrol strain B1619 had strong colonization ability in tomato root, and its colonization amount could reach more than 108 CFU / g in MS plate. In pot experiment, the amount of biocontrol bacteria in root of tomato showed the trend of decreasing firstly and then increasing and then stabilizing in conventional soil or after inoculation with R. solanacearum. On the fifteenth day, the colonization rate reached 106CFU / g root. After the tomato was planted, B1619 promoted the fungi and actinomycetes in the rhizospheric soil, while the bacteria showed the first to promote the post-inhibitory effect. In addition, B1619 could increase soil invertase activity and inhibit soil urease activity. On the 15th day after B1619 application, the activity of invertase reached 226.3 mg / g, 88 mg / g higher than that of the control. The soil urease activity was similar to that of the control On the contrary, a downward trend.