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目的 探讨苦杏仁甙对体外高氧暴露早产鼠肺泡Ⅱ型细胞 (type2alveolarepithelialcell,AECⅡ)的保护作用机制。方法 原代培养早产鼠AECⅡ,建立高氧细胞模型,采用MTT比色法、流式细胞术、免疫印迹(Westernblot)、逆转录聚合酶链反应 (RT PCR)等方法,观察苦杏仁甙对高氧暴露早产鼠AECⅡ增殖及表面活性物质蛋白 (surfactantassociatedprotein, SP)mRNA表达的影响。结果 高氧暴露导致早产鼠AECⅡ增殖抑制,AECⅡSPsmRNA表达降低。MTT试验显示,苦杏仁甙50~200μmol/L时呈剂量依赖方式促进早产鼠AECⅡ细胞增殖, 200μmol/L浓度时,其作用最强, 400μmol/L浓度时反而呈抑制作用。200μmol/L苦杏仁甙可显著促进体外高氧暴露AECⅡ增殖,提高其SPmRNA表达水平。结论 高氧暴露导致早产鼠AECⅡ增殖抑制及SPmRNA表达降低, 200μmol/L苦杏仁甙对体外高氧暴露的早产鼠AECⅡ有一定保护作用。
Objective To investigate the protective effect of amygdalin on type 2 alveolar epithelial cells (AEC Ⅱ) in premature rats exposed to hyperoxia. Methods AEC Ⅱ was cultured in primary culture of neonatal rats. The models of hyperoxia cells were established. MTT colorimetric assay, flow cytometry, Western blot and reverse transcription polymerase chain reaction (RT PCR) were used to observe the effects of amygdalin Effects of Oxygen Exposure on AEC Ⅱ Proliferation and Expression of Survivin Protein (SP) in Preterm Rats. Results Exposure to hyperoxia led to inhibition of proliferation of AEC Ⅱ in preterm rats and decreased expression of AEC Ⅱ SP mRNA. MTT assay showed that amyrin at 50 ~ 200μmol / L promoted the proliferation of premature AECⅡ cells in a dose-dependent manner. At the concentration of 200μmol / L, the effect was the strongest, while the inhibitory effect was observed at the concentration of 400μmol / L. 200μmol / L amygdalin can significantly promote the proliferation of AEC Ⅱ exposed to hyperoxia in vitro and increase the expression of SP mRNA. Conclusion Hyperoxia exposure can inhibit the proliferation of preeclampsia AEC Ⅱ and decrease the expression of SP mRNA. Pretreatment with 200 μmol / L of amygdalin can protect AEC Ⅱ cells from hyperoxia-exposed preterm rats.