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目的:研究黑果枸杞根部的内生真菌E21次生代谢产物。方法:利用固体大米培养基对该菌株进行发酵培养,采用柱色谱以及重结晶等方法分离化合物。结果:经形态学特征及ITS序列分析结果鉴定菌株E21为白耙齿菌Irpex lacteus。从其发酵物中分离得到4个化合物,分别为monacolin K,dehydromonaco-lin K,5α,8α-环二氧麦角甾-6,22-二烯-3β-醇和麦角甾醇,其中monacolin K和dehydromonacolin K首次从白耙齿菌Irpex lacteus中分离得到。结论:monacolin K对革兰阴性菌和革兰阳性菌都具有很好的抑菌活性,而dehydromonacolin K仅对枯草芽孢杆菌具有一定的抑菌活性。
Objective: To study the secondary metabolites of endophytic fungi E21 in Lycium barbarum. Methods: The strain was cultured in solid rice medium and the compounds were separated by column chromatography and recrystallization. Results: The strain E21 was identified as Irpex lacteus by morphological characteristics and ITS sequence analysis. Four compounds were isolated from their fermentations, monacolin K, dehydromonaco-lin K, 5α, 8α-cyclotericotetra-6,22-dien-3β-ol and ergosterol, among which monacolin K and dehydromonacolin K Isolated from Irpex lacteus for the first time. CONCLUSION: Monacolin K has good antibacterial activity against Gram-negative and Gram-positive bacteria, while dehydromonacolin K has bacteriostatic activity against Bacillus subtilis only.