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目的研究缬沙坦对高脂模型大鼠动脉粥样硬化及炎性因子水平的影响。方法通过建立高脂血症动脉粥样硬化大鼠模型,动态、系统地监测血脂及高敏C反应蛋白(hs-CRP)、肿瘤坏死因子(TNF)-α、白介素(IL)-6和IL-8的水平,同时采用Trizol法提取RNA,用RT-PCR法测量主动脉中hs-CRP mRNA、TNF-αmRNA和IL-6 mRNA的表达,并且观察粥样硬化动脉内膜形态学的改变。结果缬沙坦药物治疗后,血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平较高脂模型组有所降低,但差异无统计学意义(P>0.05)。高脂模型组的hs-CRP、TNF-α、IL-6、IL-8均升高,且与正常对照组比较差异具有统计学意义(P<0.05)。应用缬沙坦治疗10 w后,hs-CRP和IL-6的水平均降低,与高脂模型组比较差异有统计学意义(P<0.05);TNF-α和IL-8的水平均降低,但与高脂模型组比较差异无统计学意义(P>0.05)。应用缬沙坦治疗10 w后,hs-CRP mRNA,TNF-αmRNA和IL-6 mRNA表达均降低,与高脂模型组比较差异有统计学意义(P<0.05)。缬沙坦药物干预组内膜病变较高脂模型比较有所减轻。结论缬沙坦能够有效地降压的同时,能减少部分炎性因子释放的作用,发挥其抗动脉粥样硬化的作用。
Objective To investigate the effects of valsartan on the levels of atherosclerosis and inflammatory cytokines in hyperlipidemic rats. Methods The hyperlipidemic atherosclerosis rat models were established to monitor the levels of serum lipids, hs-CRP, TNF-α, IL-6 and IL- 8 mRNA and RNA were extracted by Trizol method. The expressions of hs-CRP mRNA, TNF-αmRNA and IL-6 mRNA in the aorta were measured by RT-PCR and the changes of intima-morphology in atherosclerotic arteries were observed. Results After valsartan treatment, serum total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) levels were lower than those in the high fat model group, but the difference was not statistically significant (P> 0.05). The levels of hs-CRP, TNF-α, IL-6 and IL-8 in high-fat model group were all significantly higher than those in normal control group (P <0.05). The levels of hs-CRP and IL-6 decreased after treatment with valsartan for 10 w. The levels of TNF-α and IL-8 in the model group were significantly lower than those in the model group (P <0.05) However, there was no significant difference between the model group and the model group (P> 0.05). After treatment with valsartan for 10 w, the expressions of hs-CRP mRNA, TNF-αmRNA and IL-6 mRNA decreased, with statistical significance (P <0.05). Valsartan drug intervention group compared with the high-fat model of endometrial lesions lighter. Conclusion Valsartan can effectively depressurize blood pressure, reduce the release of some inflammatory factors and exert its anti-atherosclerosis effect.