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在不同植物生长调节剂组合、不同培养基条件下,探讨了马蹄金叶片离体培养的方法和影响因素。结果表明,含不同植物生长调节剂组合的培养基对愈伤组织的诱导频率无显著影响,出愈率均达90%～100%;以MS或B5培养基作诱导愈伤组织的基本培养基效果较好;将叶片接种到MS+2mg/L6-BA的诱导培养基中诱导愈伤组织,再用B5+1.5mg/L6-BA+0.5mg/LZT+0.2mg/Lα-NAA作分化培养基出苗效果最佳,愈伤组织分化频率高达20%。
In different plant growth regulators combination, different medium conditions, discussed in vitro methods and influencing factors of Dichondra repens. The results showed that the medium containing different combinations of plant growth regulators had no significant effect on the induction frequency of callus, and the recovery rates reached 90% -100%. MS or B5 medium was used as the basic medium for callus induction And the effect was better. The callus was inoculated into the induction medium of MS + 2mg / L 6-BA and then cultured with B5 + 1.5mg / L 6-BA + 0.5mg / LZT + 0.2mg / Lα-NAA Based on the best seedling emergence, callus differentiation frequency of up to 20%.