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目的:研究温郁金醚提物中二萜类化合物C对人胃癌细胞(SGC-7901)增殖的抑制作用及对胃癌细胞中Bcl-2、Bax表达的影响。方法:以含10%胎牛血清的RPMI1640为培养基,置37℃、体积分数为5%的CO2培养箱中培养SGC-7901细胞;将温郁金醚提物中二萜类化合物C[1]溶于二甲基亚砜(Dimethyl sulfoxide,DM-SO),配成10mg/mL母液;以β-榄香烯、顺铂(cis-platinum complexes,DDP)为阳性对照药物,采用噻唑蓝(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT)比色法检测两种药物在0、10、30、50、70μg/mL浓度(24、48、72h)对SGC-7901细胞增殖的影响;用Western Blot杂交法检测经温郁金二萜类化合物C作用后的人胃癌细胞SGC-7901中凋亡相关蛋白Bcl-2、Bax表达影响。结果:MTT法显示β-榄香烯、顺铂、化合物C48h对SGC-7901的半数抑制率(IC50)为分别为55.27、38.01、30.14μg/mL,在较低浓度时的抑制率与阳性对照组相似,在50,70μg/mL浓度时的抑制率与两对照组相比差异有统计学意义(P<0.05);且具有一定的时间依赖性;Western Blot提示温郁金二萜类化合物C可上调促凋亡蛋白Bax的表达、下调抑制凋亡蛋白Bcl-2的表达。结论:温郁金醚提物中二萜类化合物C对胃癌SGC-7901细胞的增殖有显著的抑制作用,高浓度时其抑癌率较β-榄香烯、顺铂的明显,其作用具有一定的时间和浓度依赖性;通过影响Bcl-2/Bax的表达是其发挥抗肿瘤作用的可能机制之一。
Objective: To study the inhibitory effect of diterpene C on the proliferation of human gastric cancer cell line SGC-7901 and its effect on the expression of Bcl-2 and Bax in gastric cancer cells. Methods: SGC-7901 cells were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum at 37 ℃ in 5% CO 2 incubator. The diterpenoid C [1] In dimethylsulfoxide (DM-SO), dubbed 10mg / mL mother liquor; with β-elemene and cisplatin (DDP) as positive control drugs, (4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) -7901 cell proliferation. The effect of the apoptosis-related proteins Bcl-2 and Bax on the apoptosis of human gastric cancer cell SGC-7901 was observed by Western blotting. Results: MTT assay showed that the IC50 values of β-elemene, cisplatin and compound C48h on SGC-7901 were 55.27, 38.01 and 30.14 μg / mL, respectively. The inhibitory rates at low concentrations were similar to those of positive control Group, the inhibition rates at 50 and 70 μg / mL were significantly different from those of the two control groups (P <0.05), and had a certain time-dependent manner. Western Blot suggested that the warm-diterpenoid C could up-regulate The expression of pro-apoptotic protein Bax, down-regulated the expression of Bcl-2. CONCLUSION: Diterpenoid C in the extract of V. yunnanensis significantly inhibits the proliferation of gastric cancer cell line SGC-7901, and its inhibitory rate is higher than that of β-elemene and cisplatin at high concentration. Time and concentration dependence. It is one of the possible mechanisms by which Bcl-2 / Bax can exert antitumor effects.