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早期研究表明与正常儿童相比,维生素A缺乏儿童血浆尿素氮(UN)与氨基氮(AN)水平低,并且与肌酸酐(Cr)相关的尿氨态氮(Am-N)24小时排泄量较高。本实验旨在研制一种合适的维生素A缺乏的动物模型,其氮代谢产物的改变应与儿童中观察到的相似;并且进行了尿素循环途径的关键酶——氨甲酰磷酸合成酶-1(CPS-1)、鸟氨酸转氨甲酰酶(OTC),精氨酸酶的活性及其中间产物的研究,以便更好地认识上述改变的生化机制。实验中将20只断乳Wistar/NIH雌性大鼠随机分为维生素A缺乏组与对照组,每组10只。维生素A缺乏组:大鼠饲喂含20%酪蛋
Early studies showed that children with vitamin A deficiency had lower levels of plasma urea nitrogen (NUN) and amino nitrogen (AN) compared with normal children, and urinary ammonia nitrogen (Am-N) associated with creatinine (Cr) Higher. The aim of this experiment was to develop a suitable animal model of vitamin A deficiency in which changes in nitrogen metabolism should be similar to those observed in children; and carbamoyl-phosphate synthetase-1, a key enzyme in the urea cycle, (CPS-1), ornithine transcarbamylase (OTC), arginase activity and its intermediates in order to better understand the biochemical mechanism of the above changes. Twenty experimental weanling Wistar / NIH female rats were randomly divided into vitamin A deficiency group and control group, with 10 mice in each group. Vitamin A Deficiency Group: Rats fed 20% casein