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目的:探讨CYP1B1敲除在小鼠骨髓移植中生存情况中的作用。方法:20只10周龄雄性C57BL/6J小鼠用8.25Gy 60 Co全身照射后,随机分为两组(KO组和WT组),于3h内分别尾静脉注射WT小鼠骨髓和CYP1B1敲除(CYP1B1-/-)小鼠骨髓,观察两组小鼠存活情况以及移植物抗宿主病(GVHD)的情况。结果:8.25Gy60 Co照射后,CYP1B1-/-→WT(KO)组小鼠骨髓移植后平均生存时间长于WT→WT(WT)组小鼠骨髓移植平均生存时间,GVHD评分始终低于WT组,小鼠体重增长率始终高于WT组小鼠,辐射后小鼠体重增量显著高于WT组小鼠,P<0.05。结论:CYP1B1敲除在小鼠骨髓移植中有延长平均生存时间、增加小鼠体重、改善GVHD的效果,对骨髓移植后的小鼠恢复有一定保护作用。
Objective: To investigate the role of CYP1B1 knockout in the survival of mouse bone marrow transplantation. Methods: Twenty male C57BL / 6J mice, aged 10 weeks, were randomly divided into two groups (KO group and WT group) with 8.25Gy 60Co whole body irradiation. The bone marrow and CYP1B1 knockout (CYP1B1 - / -) mice bone marrow to observe the survival of two groups of mice and graft-versus-host disease (GVHD). Results: After 8.25Gy60Co irradiation, the average survival time of bone marrow transplantation in mice of CYP1B1 - / - → WT (KO) group was longer than that of WT → WT group (WT) mice. The GVHD score was always lower than WT group, The body weight growth rate of mice was always higher than that of WT mice. The body weight gain of mice after radiation was significantly higher than that of WT mice (P <0.05). CONCLUSION: CYP1B1 knock-out can prolong the mean survival time, increase the body weight of mice and improve the effect of GVHD in mice bone marrow transplantation, and have a protective effect on the recovery of mice after bone marrow transplantation.