Matsamura 等应用放射免疫测定法,发现成人胃窦含有β-内啡肽样物质。Polak 等的免疫组织化学研究,见到成人胃窦粘膜中有脑啡肽样免疫反应细胞,但未发现内啡肽免疫阳性细胞。我们用免疫组织化学法,对人胚胎胃窦是否有β-内啡肽细胞进行了观察。标本为25例3～10月胚胎和5例1～6天新生儿的胃窦前壁,10%福尔马林或 Bouin 液固定,石蜡包埋,切片厚5μm。按 Sternberger PAP法显示β-内啡肽细胞(β-内啡肽抗血清由第二军医大学生理学教研室提供)。用 PBS 分别代替Ⅰ、Ⅱ和Ⅲ抗血清作方法对照,并用人胚胎垂体作β-内啡肽阳性细胞对照。 Matsamura et al. Used radioimmunoassay and found that the adult gastric antrum contains beta-endorphin-like substances. Immunohistochemical studies by Polak et al. Showed that there were enkephalin-like immunoreactive cells in the gastric mucosa of an adult but no endorphin-immunopositive cells were found. We used immunohistochemistry to examine whether there is beta-endorphin in human gastric antrum. Specimens were 25 anterior embryos from March to October and 5 anterior wall of the antrum of newborns from 1 to 6 days. They were fixed in 10% formalin or Bouin solution and embedded in paraffin. The sections were 5 μm thick. The β-endorphin cells were shown by the Sternberger PAP method (β-endorphin antiserum was provided by the Department of Physiology, Second Military Medical University). The mice were treated with PBS instead of the antiserums of Ⅰ, Ⅱ and Ⅲ respectively. The pituitary of human embryo was used as control for β-endorphin positive cells.