论文部分内容阅读
目的对文献已有报道的胎盘和白细胞之间甲基化水平有差异的DNA片段在大样本中国人群进行验证,探索可能用于中国人群的DNA甲基化标记物。方法以母亲胎盘胎儿面和外周血白细胞为研究对象,采用甲基化敏感性限制性内切酶酶切,检测母亲外周血白细胞和母亲胎盘胎儿面甲基化状态的差别,并在孕妇外周血血浆中进行检测,寻找可能的标记。结果通过甲基化敏感性酶切分析的方法,已报道的标记物中,无一能作为临床可用的非侵入性产前诊断唐氏综合征的胎儿DNA甲基化标记。结论通过Hpa Ⅱ-MspⅠ酶切法分析区分母儿DNA的方法需要进一步探索。已报道的标记物中,无一能作为临床可用的非侵入性产前诊断唐氏综合征的胎儿DNA甲基化标记。
OBJECTIVE: To identify DNA methylation markers that may be used in Chinese population in large populations of Chinese population for DNA fragments that have been reported to differ in methylation level between placenta and leukocytes. Methods The fetal placenta and peripheral blood leucocytes were used as the research objects. Methylation-sensitive restriction endonuclease digestion was used to detect the fetal methylation status of mothers’ peripheral white blood cells and their mothers. The peripheral blood The plasma is tested for possible markers. Results By methylation-sensitive enzyme digestion analysis, none of the reported markers could be used as a marker of fetal DNA methylation in clinically available noninvasive prenatal diagnosis of Down’s syndrome. Conclusion Hpa Ⅱ-Msp Ⅰ digestion method to distinguish between mother and child DNA needs further exploration. None of the reported markers have been identified as fetal DNA methylation markers for clinically useful noninvasive prenatal diagnosis of Down’s syndrome.