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目的:探讨益肾汤对链脲佐菌素诱导的糖尿病大鼠肾组织NF-κB的影响。方法:将41只大鼠随机分为4组:正常对照组、糖尿病模型组、糖尿病缬沙坦治疗组、糖尿病益肾汤治疗组。干预8 w后检测空腹血糖、血肌酐、血尿素氮、24 h尿总蛋白、尿白蛋白排泄率,免疫组化测定肾组织NF-κB表达。结果:与模型组比较,益肾汤及缬沙坦治疗组尿白蛋白排泄率均显著降低(P<0.05)、肾组织NF-κB的表达受到抑制(P<0.05);肾组织NF-κB表达量与空腹血糖、24 h尿总蛋白、尿白蛋白排泄率呈显著正相关(r与P值分别为:r=0.63,P=0.001;r=0.53,P 0.007;r=0.84,P<0.001)。结论:益肾汤可减少尿白蛋白排出、减少肾脏NF-κB的表达;益肾汤对糖尿病大鼠肾脏的保护作用与其下调肾脏NF-κB的活化有关。
Objective: To investigate the effect of Yishen Decoction on the renal tissue NF-κB in streptozotocin-induced diabetic rats. Methods: Forty-one rats were randomly divided into 4 groups: normal control group, diabetic model group, diabetic valsartan treatment group and Diabetes Yishen Decoction treatment group. After 8 weeks of intervention, the fasting blood glucose, serum creatinine, blood urea nitrogen, 24 h urine total protein and urinary albumin excretion rate were measured, and the expression of NF-κB in renal tissue was detected by immunohistochemistry. Results: Compared with the model group, the urinary albumin excretion rate of Yishen decoction and valsartan treatment group were significantly decreased (P <0.05), while the expression of NF-κB in renal tissue was inhibited (P <0.05) The expression level was positively correlated with fasting blood glucose, 24 h urinary total protein and urinary albumin excretion rate (r = 0.63, P = 0.001; r = 0.53, P 0.007, r = 0.84, P < 0.001). Conclusion: Yishen Decoction can reduce urinary albumin excretion and reduce the expression of NF-κB in kidney. The protective effect of Yishen Decoction on kidneys in diabetic rats is related to the decrease of renal NF-κB activation.