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枯草芽孢杆菌(Bacillus subtilis)LL18-4菌株分泌物中的类细菌素通过硫酸铵盐析法粗提后,经过Sephadex G-75、DEAE-Sephadex A 50和Sephacryl S-200HR分步柱层析,SDS-PAGE电泳检测到单一蛋白质条带,分子量约为60 kD.该类细菌素在酸性条件下及80%饱和度的硫酸铵溶液中具有较高的稳定性,可4℃放置3个月仍保持80%以上活性;对有机溶剂有一定的耐受性;Mg~(2+)能提高其抑菌活性.指数增长前期添加柑桔绿霉(Peniicilbumdiigitatum)病原真菌的培养液可以提高该类细菌素的分泌量.
The bacteriocins in the secretions of Bacillus subtilis strain LL18-4 were crudely purified by ammonium sulfate salting-out and were separated by Sephadex G-75, DEAE-Sephadex A 50 and Sephacryl S-200HR, A single protein band was detected by SDS-PAGE electrophoresis and had a molecular weight of about 60 kD. The bacteriocins showed high stability under acidic conditions and 80% saturation of ammonium sulfate solution and were still stable at 4 ° C for 3 months Keep more than 80% activity, have certain tolerance to organic solvents, and Mg 2+ can increase its antibacterial activity.Improvement of exponentially increasing culture medium of Penicillium digeitum pathogenic fungi can increase the activity of this kind of bacteria The secretion of prime.