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目的探讨舒血宁注射液对脓毒症损害的大鼠心肌是否具有保护作用。方法将30只SD大鼠随机分为假手术组(Sham组)、脓毒症组(CLP组)与舒血宁注射液组(SXN组),CLP组与SXN组采用大鼠盲肠结扎穿孔术制造脓毒症模型。SXN组于盲肠结扎穿孔术前1h经腹腔注射0.3ml/kg舒血宁注射液,Sham组和CLP组于相同时间点给予0.3ml/kg生理盐水。术后6h时经腹主动脉取血行肌钙蛋白T(TnT)、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β检测;取部分心肌匀浆,制成10%心肌组织悬液,测定超氧化物歧化酶(SOD)活性与丙二醛(MDA)含量;部分心肌行H-E染色观察病理变化。结果与CLP组比较,舒血宁可显著降低CLP后血浆TnT、TNF-α与IL-1β水平以及心肌组织MDA含量,并使心肌SOD活性增强(P<0.05或0.01);组织学观察可见舒血宁可明显改善脓毒症组大鼠心肌充血、水肿及炎性细胞浸润等病理改变。结论舒血宁对脓毒症大鼠心肌损伤具有保护作用,其机制可能与其对心肌氧自由基的清除作用及对炎性介质的影响有关。
Objective To investigate whether shuxuening injection can protect myocardium from sepsis in rats. Methods Thirty SD rats were randomly divided into sham operation group (Sham group), sepsis group (CLP group) and Shuxuening injection group (SXN group). Rats in CLP group and SXN group received cecal ligation and puncture Make sepsis model. SXN group was injected intraperitoneally with 0.3ml / kg Shuxuening injection 1h before cecal ligation and perfusion. Sham group and CLP group were given 0.3ml / kg normal saline at the same time point. Six hours after operation, blood was taken from the abdominal aorta for detection of TnT, TNF-α and IL-1β; some of the myocardium was homogenized to make 10% Fluid, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were measured. The part of myocardium was observed with HE staining to observe the pathological changes. Results Compared with CLP group, Shuxuening significantly reduced plasma levels of TnT, TNF-α and IL-1β, and myocardial MDA levels, and enhanced myocardial SOD activity (P <0.05 or 0.01). Histological observation showed that Shu Xue Ning Would rather improve the sepsis rat myocardial congestion, edema and inflammatory cell infiltration and other pathological changes. Conclusion Shuxuening has a protective effect on myocardial injury in septic rats, and its mechanism may be related to the scavenging effect on myocardial oxygen free radicals and its effect on inflammatory mediators.