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本文报道以ＭＴＴ法检测ＬＡＫ细胞冻融液对肿瘤细胞直接杀伤作用，确立检测过程中的某些最适条件。发现以Ｋ５６２细胞和ＮＫＭ－４５细胞为靶细胞时，试验最适细胞浓度分别为２×１０６／ｍｌ和１．５×１０６／ｍｌ。为阻断检测过程中肿瘤细胞增殖对结果的影响，需加入终浓度为ｌｕｇ／ｍｌ的放线菌素Ｄ。实验结果提示，ＬＡＫ细胞可能通过释放某些杀伤分子介导对肿瘤细胞的直接杀伤作用。
This paper reports the direct killing effect of freeze-thaw fluid from LAK cells on tumor cells by MTT assay and establishes some of the optimal conditions for the detection process. When K562 cells and NKM-45 cells were used as target cells, the optimal cell concentrations for the assay were 2×10 6 /ml and 1.5×10 6 /ml, respectively. In order to block the effect of tumor cell proliferation on the results during the assay, actinomycin D was added at a final concentration of lug/ml. The experimental results suggest that LAK cells may mediate direct killing of tumor cells by releasing certain killer molecules.