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[目的]观察氟性骨损伤患者血清dickkopf相关蛋白1(DKK-1)水平,分析氟性骨损伤的发生与DKK-1表达的相关性。[方法]采用自行设计的“氟与健康”量表对氟暴露人群进行问卷调查,在调查对象知情同意的前提下,采集静脉血及随机中段尿用于检测血氟、尿氟及血清中DKK-1蛋白浓度,并对其进行氟斑牙筛检及前臂正位X线检查。根据血氟浓度、氟斑牙及氟性骨损伤程度将调查对象分别分为不同氟负荷组、氟斑牙与否组及不同程度骨损伤组,分析不同组别之间DKK-1的差异。[结果]血清DKK-1在低、中、高氟负荷组的浓度分别为(19.60±0.52)、(17.74±0.80)、(14.31±1.20)μg/L,随氟负荷的增高血清DKK-1浓度依次降低,差异有统计学意义(P<0.05)。无氟斑牙组与氟斑牙组人群血清DKK-1水平差异无统计学意义(P>0.05)。无、轻、中、重度氟性骨损伤组血清DKK-1浓度分别为(19.16±0.76)、(15.86±1.35)、(13.27±0.45)、(12.42±0.04)μg/L;骨损伤组血清DKK-1浓度均低于无骨损伤组,差异有统计学意义(P<0.05)。[结论]氟性骨损伤患者血清DKK-1表达水平较低,提示高氟暴露可以致机体DKK-1蛋白表达下调,导致氟性骨损伤的发生。
[Objective] To observe the serum level of dkkk-1 (DKK-1) in patients with fluorosis bone injury and analyze the correlation between the expression of DKK-1 and the occurrence of fluorosis bone injury. [Methods] A self-designed “fluorine and health” scale was used to survey the subjects exposed to fluoride. Under the informed consent of the subjects, venous blood and random middle urine were collected to detect blood fluoride, urine fluoride and serum In DKK-1 protein concentration, and its dental fluorosis screening and forearm X-ray examination. According to the levels of blood fluoride, dental fluorosis and fluorosis, the subjects were divided into different fluoride load group, dental fluorosis group and different degrees of bone injury group, and the differences of DKK-1 among different groups were analyzed. [Results] Serum DKK-1 levels were (19.60 ± 0.52), (17.74 ± 0.80) and (14.31 ± 1.20) μg / L in low, medium and high fluoride group, The concentration decreased in turn, the difference was statistically significant (P <0.05). There were no significant differences in serum DKK-1 levels between PFD group and dental fluorosis group (P> 0.05). The serum levels of DKK-1 in the groups of no, mild, moderate and severe fluorosis were (19.16 ± 0.76), (15.86 ± 1.35), (13.27 ± 0.45) and (12.42 ± 0.04) μg / L, respectively DKK-1 concentrations were lower than those without bone injury, the difference was statistically significant (P <0.05). [Conclusion] The low level of serum DKK-1 expression in patients with fluorosis bone injury suggests that high fluoride exposure can down-regulate the expression of DKK-1 and lead to the occurrence of fluorosis bone injury.