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同步辐射红外显微光谱技术凭借其超高亮度和高空间分辨率的优势,已经在多学科领域中取得了大量的研究成果。特别是在生物医学领域,同步辐射红外显微光谱可以对无染色、无标记的生物样品进行无损检测并可获得生物分子的大量结构信息,因此得到广泛应用。随着同步辐射红外显微光谱技术的发展,生物化学家和光谱学家已经将研究的重点从组织层次的红外光谱成像(组织红外光谱成像)扩展到细胞层次的红外光谱成像(细胞红外光谱成像),并在近十年的研究中取得了大量的研究成果,但同时也暴露出一些问题,例如(1)细胞或介质中的水在红外光谱酰胺Ⅰ谱带具有很强的吸收;(2)不平整的细胞表面会导致红外光谱中产生Mie散射;(3)细胞红外光谱的复杂性和不确定性会影响数据分析的有效性和准确性。另一方面,生化学家和光谱学家也为解决这些问题采取了许多有用的策略。因此,本综述首先从样品制备、实验设计以及数据分析等方面对最近十年来细胞同步辐射红外光谱成像技术取得的成果进行了总结,随后介绍了目前细胞红外成像技术面临的问题以及解决策略。我们相信,通过多束同步辐射红外光与焦平面阵列(FPA)探测器的结合,同步辐射红外光谱成像技术在对细胞的结构和功能研究中以及其他领域不同材料的研究中都会逐步显示出独特的作用。
Synchrotron radiation (IR) microspectroscopy has gained a great deal of research in multidisciplinary fields due to its superior brightness and spatial resolution. Especially in the field of biomedicine, synchrotron radiation (IR) spectroscopy has been widely used due to the non-destructive detection of non-stained, unlabeled biological samples and the availability of a large amount of structural information on biomolecules. With the development of synchrotron radiation (IR) microspectroscopy, biochemists and spectroscopists have extended the research focus from tissue-level IR spectroscopy (TIR imaging) to cellular-level IR spectroscopy (CTIS ), And in the past ten years of research has made a lot of research results, but also revealed some problems, such as (1) cell or medium water in the infrared spectrum amide Ⅰ band has a strong absorption; (2 ) Uneven cell surfaces can result in Mie scattering in the infrared spectrum; (3) The complexity and uncertainty of the cellular infrared spectra can affect the validity and accuracy of the data analysis. On the other hand, biochemists and spectroscopists have also taken many useful strategies to solve these problems. Therefore, this review summarizes the results of CSPIR imaging in the recent decade from the aspects of sample preparation, experimental design and data analysis, and then introduces the current problems and countermeasures of Cellular Infrared Imaging technology. We believe that by combining multiple synchrotron radiation infrared with a FPA detector, synchrotron radiation infrared imaging techniques will gradually show uniqueness in the study of the structure and function of cells and in various fields of other materials Role.