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1979年我们采用根尖细胞染色体压片法,对34个饲用南瓜材料进行了染色体的观察经20批179个根尖的检查,确定饲用南瓜染色体数二倍体(2n)为40个,其中黄15—5饲用南瓜染色体为80个,为四倍体。现将我们观察的方法和步骤介绍如下: 1、种子发芽;最好使种子在恒温箱中萌发;也可用木箱砂土培养,靠自然温度萌发。 2、取材;当幼根长至2—3厘米时,在上午10—11时,切取根尖1厘米,装入试管内进行处理。如果是砂土培养的根尖,必须将根尖上的泥砂洗净。
In 1979, we used root tip cell chromosome pressing method to observe the chromosomes of 34 feed pumpkin materials. After the examination of 20 rootlets and 179 root tips, the number of diploid chromosomes (2n) in feeding pumpkin was 40, Among them, there are 80 chromosomes in yellow 15-5 feed pumpkin, tetraploid. Now we observe the methods and steps are described below: 1, the seeds germinate; the best seed germination in the incubator; also available sand culture wooden box, germination by natural temperature. 2, drawn; when the root length to 2-3 cm, 10-11 in the morning, cut root tip 1 cm, into the test tube for processing. If it is the root tip of sand culture, the sand on the root tip must be washed.