论文部分内容阅读
目的甲型H1N1流感病毒A/California/7/2009分别与A/Brisbane/10/07和A/ShenZhen/406H/06共感染小型香猪,预测甲流病毒在与季流H3N2病毒/甲流病毒与禽流感病毒共感染时是否会发生变异。方法分别将A/California/7/2009(CA7)与A/Brisbane/10/07(H3N2),A/California/7/2009与A/Shenzhen/406H/06(H5N1)对5～6月龄小型猪共感染,小型猪经复方氯胺酮0.1 mL/kg麻醉后进行滴鼻感染,感染后第5天安乐死动物,取动物肺组织作病毒测序分析。结果 A/California/7/2009(CA7)与A/Brisbane/10/07(H3N2)共感染后,A/California/7/2009病毒PB1基因993位G→A突变,PA基因1659位G→A突变,没有氨基酸的变异。A/California/7/2009与A/Shenzhen/406H/06(H5N1)共感染后A/California/7/2009病毒PB2基因1711位T→C突变。碱基的突变未引起氨基酸的变异。结论 A/California/7/2009(CA7)与A/Brisbane/10/07(H3N2),A/California/7/2009与A/Shenzhen/406H/06(H5N1)共感染后在猪的体内没有发生病毒重组、变异。
Objective To co-infect small swine pigs with A / California / 7/2009 with A / Brisbane / 10/07 and A / ShenZhen / 406H / 06 respectively to predict the safety of pandemic influenza virus (H1N1) Variants occur when co-infected with avian influenza virus. Methods A / California / 7/2009 (CA7) and A / Brisbane / 10/07 (H3N2), A / California / 7/2009 and A / Shenzhen / 406H / 06 Pigs were co-infected. Pigs were intranasally infected with compound ketamine (0.1 mL / kg), and the animals were euthanized on the fifth day after infection. The lung tissues of the animals were sequenced. Results After co-infection with A / California / 7/2009 (CA7) and A / Brisbane / 10/07 (H3N2), the A → California / 7/2009 G3 mutation of PB1 gene at position 993 and G → A Mutation, no amino acid variation. A / California / 7/2009 A / California / 7/2009 and A / Shenzhen / 406H / 06 (H5N1) co-infected with A / California / 7/2009 virus PB2 gene 1711 T → C mutation. Mutations in the base did not cause amino acid variations. Conclusion No co-infection in pigs occurred after co-infection with A / California / 7/2009 (CA7) and A / Brisbane / 10/07 (H3N2), A / California / 7/2009 and A / Shenzhen / 406H / 06 (H5N1) Virus recombination, mutation.