论文部分内容阅读
以逆转录病毒ｐＬＸＳＬ为载体与人细胞介素２基因（ＩＬ－２）重组，用电穿孔技术将其重组子ｐＬＩＬ—２ＳＮ导入ＰＡ３１７细胞中，建立了逆转染病毒包装体系细胞ＰＡ３１７／ｐＬＩＬ—２ＳＮ。应用逆转录病毒包装体系细胞上清液去转染入肺腺癌细胞ＳＰＣ—Ａ１，经过２０天含Ｇ４１８培液的筛选式培养，历经了５０代的传代培养，获得了转白细胞介素２基因的人肺腺癌细胞株ＳＰＣ－Ａ１／ＩＬ－２。该细胞（ＳＰＣ－Ａ１／ＩＬ－２）经ＰＣＲ技术验证外源性目的基因（ＩＬ－２ｇｅｎｅ）导入细胞内，且证明该细胞能表达ＩＬ—２基因（有ＩＬ－２的分泌）。我们研究的目的是对肿瘤细胞的特异性抗原修饰、对肿瘤细胞进行处理。试图建立肿瘤疫苗。
Recombinant pLXSL was used as a vector to reconstitute IL-2 gene. The recombinant plasmid pLIL-2SN was introduced into PA317 cells by electroporation to establish a retroviral packaging system cell PA317/pLIL. 2SN. The cell supernatant of retrovirus packaging system was transfected into lung adenocarcinoma cell SPC-A1. After 20 days of selective culture containing G418 culture medium, 50 days of subculture were used to obtain the transleukin 2 gene. Human lung adenocarcinoma cell line SPC-A1/IL-2. This cell (SPC-A1/IL-2) was verified by PCR to introduce into the cell an exogenous gene of interest (IL-2gene), and it was demonstrated that the cell can express the IL-2 gene (with the secretion of IL-2). The purpose of our study was to specifically modify tumor cells and treat tumor cells. Trying to build a tumor vaccine.