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利用Solt-Faber法制备大鼠肝癌初期模型,同时以100 mg.kg-1大豆异黄酮和皂甙干预42 d。然后处死动物,制备肝匀浆及肝线粒体提取液。以比色法测定丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、γ-谷酰胺转肽酶(γ-GT)、谷胱甘肽-S-转移酶(GST)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)含量。结果表明,大豆异黄酮和皂甙能降低肝癌发生初期大鼠肝脏ALT、AST、γ-GT和GST活性,升高肝脏及肝线粒体SOD、CAT、GSH-PX活性和降低MDA水平。提示,大豆异黄酮和皂甙具有减轻肝癌发生初期大鼠肝细胞损伤,降低其氧化应激的作用。此作用有助于预防肝癌的发生。
The initial model of rat hepatocarcinoma was prepared by Solt-Faber method, while the intervention of 100 mg.kg-1 soy isoflavone and saponin for 42 days. Animals were then sacrificed to prepare liver homogenates and liver mitochondria extracts. The contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (γ-GT) and glutathione S-transferase ), Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) activity and malondialdehyde (MDA) content were measured. The results showed that soy isoflavones and saponins could reduce the hepatic ALT, AST, γ-GT and GST activities, increase the activities of SOD, CAT, GSH-PX and decrease the levels of MDA in the liver and liver mitochondria. Tip, soy isoflavones and saponins have the role of reducing hepatocellular injury and reducing their oxidative stress in primary hepatocellular carcinoma. This effect can help prevent the occurrence of liver cancer.