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目的探讨组蛋白去乙酰化酶抑制剂MS-275对口腔鳞状细胞癌(Tca-8113)生长抑制情况以及促进凋亡的作用。方法采用0、0.5、1、2、4、8μmol/L MS-275对口腔鳞状细胞癌进行干预,倒置相差显微镜观察细胞形态改变;MTT法检测细胞增殖活性;Annexin-V-FITC/PI双染流式细胞仪定量检测细胞凋亡,流式细胞仪分析细胞周期。结果①倒置相差显微镜下观察到对照组细胞呈多边形,贴壁,生长活跃;实验组细胞形态改变明显,大部分细胞核固缩,细胞变小、变圆、脱壁。②细胞生长曲线显示,随MS-275浓度增加、时间延长,Tca-8113细胞生长明显受到抑制,各实验组细胞生长抑制率与对照组相比,P<0.05,差别有统计学意义。③2、4μmol/L MS-275作用48h,细胞总凋亡率分别为41.28%和53.71%,细胞周期阻滞于G0/G1期,与对照组相比,差别有统计学意义。结论 MS-275体外抑制口腔鳞状细胞癌生长,呈时间-剂量依赖性,并促进细胞凋亡,阻滞细胞周期。
Objective To investigate the inhibitory effect of histone deacetylase inhibitor MS-275 on the growth of oral squamous cell carcinoma (Tca-8113) and its role in promoting apoptosis. Methods Oral squamous cell carcinoma was treated with 0, 0.5, 1, 2, 4 and 8μmol / L MS-275. Morphological changes were observed by inverted phase contrast microscope. Cell proliferation was measured by MTT assay. Annexin-V-FITC / Flow cytometry was used to detect apoptosis and flow cytometry was used to analyze cell cycle. Results ① The cells in the control group showed polygonal, adherent and active growth under the inverted phase contrast microscope. The morphological changes of the cells in the experimental group were obvious. Most of the cells were pyknotic, the cells became smaller, rounded and detached. ② The cell growth curve showed that the growth of Tca-8113 cells was obviously inhibited with the increase of MS-275 concentration and the prolonged time. Compared with the control group, the growth inhibition rate of Tca-8113 cells was significantly lower than that of the control group (P <0.05). ③ The effect of 2,4 and 4μmol / L MS-275 for 48h was 41.28% and 53.71% respectively. The cell cycle arrest was in G0 / G1 phase. Compared with the control group, the difference was statistically significant. Conclusion MS-275 can inhibit the growth of oral squamous cell carcinoma in vitro in a time-and dose-dependent manner and promote cell apoptosis and arrest the cell cycle.