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目的制备聚乙二醇化尿酸酶脂质体(PULPs)并考察其活性与稳定性。方法采用逆向蒸发法制备PULPs,并考察其最适温度、最适p H、热稳定性、贮存稳定性及其抗胰蛋白酶水解的能力、抗部分金属离子及有机化合物影响的能力。结果PULPs的最适温度为40℃,最适p H为8.5;PULPs的热稳定性、贮存稳定性、抗胰蛋白酶水解能力、抗部分金属离子及有机化合物影响的能力均优于尿酸酶。结论 PULPs在增强聚乙二醇化尿酸酶活性的同时增强了稳定性。
Objective To prepare pegylated uricase liposomes (PULPs) and investigate their activity and stability. Methods PULPs were prepared by reverse evaporation. The optimum temperature, pH, thermal stability, storage stability, anti-trypsin hydrolysis and the ability to resist some metal ions and organic compounds were investigated. Results The optimum temperature of PULPs was 40 ℃, and the optimum pH was 8.5. The thermal stability, storage stability, antitrypsin hydrolysis ability, resistance to some metal ions and organic compounds of PULPs were better than those of uricase. Conclusions PULPs enhance stability while enhancing pegylated uricase activity.