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目的：探讨苯并［α］芘［Ｂ（α）Ｐ］对人胎盘绒毛膜上皮细胞中细胞色素Ｐ４５０１Ａ１（ＣＹＰ１Ａ１）活性的诱导作用．方法：离体培养人胎盘绒毛膜上皮细胞，观察Ｂ（α）Ｐ（１×１０－６ｍｏｌ／Ｌ～１×１０－５ｍｏｌ／Ｌ）诱导细胞２４ｈ以及７．５×１０－６ｍｏｌ／Ｌ的Ｂ（α）Ｐ诱导细胞１２ｈ～４８ｈ，细胞中ＡＨＨ，ＥＲＯＤ活性的变化．结果：细胞经Ｂ（α）Ｐ诱导后，ＡＨＨ，ＥＲＯＤ活性显著增加，在７．５×１０－６ｍｏｌ／Ｌ时，诱导最强．用７．５×１０－６ｍｏｌ／ＬＢ（α）Ｐ诱导细胞１２ｈ～４８ｈ，诱导的酶活性在２４ｈ达到最高．结论：Ｂ（α）Ｐ可显著诱导人胎盘绒毛膜上皮细胞中ＣＹＰ１Ａ１活性，使细胞对外来物质的活化能力明显增强．为研究吸烟及环境污染对胎儿和胎盘造成危害提供了直接依据．同时证明人胎盘绒毛膜上皮细胞可作为进一步研究胎盘中ＣＹＰ１Ａ１诱导机制的实验模型
Objective: To investigate the effect of benzo [α] pyrene [B (α) P] on the cytochrome P4501A1 (CYP1A1) activity in human placental chorionic epithelial cells. Methods: Human placenta chorionic epithelial cells were cultured in vitro. The induced cells were treated with B (α) P (1 × 10-6 mol / L ~ 1 × 10-5 mol / L) for 24 h and 7.5 × 10-6 mol / L B (Α) P induced cell 12h ~ 48h, AHH, EROD activity changes. Results: After induced by B (α) P, the activity of AHH and EROD increased remarkably, and reached the highest at 7.5 × 10-6 mol / L. The cells were induced with 7.5 × 10-6mol / L (α) P for 12h ~ 48h, the highest enzyme activity reached at 24h. CONCLUSION: B (α) P can significantly induce CYP1A1 activity in human placental chorionic epithelial cells and significantly enhance the activation of foreign substances. Provide a direct basis for studying the harmful effects of smoking and environmental pollution on the fetus and placenta. At the same time, it was proved that human placenta chorionic epithelial cells can be used as an experimental model for further studying the mechanism of CYP1A1 induction in the placenta