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采用 ＷＴＴ颜色反应法研究传染性脾肾坏死病毒（ＩＳＫＮＶ）、伴刀豆球蛋白 Ａ（ｃｏｎｃａｎａｖａｌｌｉｎ Ａ，ＣｏｎＡ）和脂多糖（ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅ，ＬＰＳ）在离体状态下对健康和ＩＳＫＮＶ感染后存活３０ｄ或６０ｄ的鳜的头肾淋巴细胞转化的影响。结果表明，ＣｏｎＡ和ＬＰＳ能促进健康级头肾淋巴细胞的转化，而对感染后存活３０ｄ或６０ｄ的鳜头肾淋巴细胞没有作用；纯化的ＩＳＫＮＶ对健康鳜头肾淋巴细胞没有促进淋巴细胞转化的作用，但对ＣｏｎＡ的作用有抑制，对感染后存活的３０ｄ或６０ｄ的鳜头肾淋巴细胞，纯化的ＩＳＫＮＶ有一定的促进转化的作用。感染存活的鳜头肾中有对ＩＳＫＮＶ的免疫记忆性Ｔ细胞。另外，用建立的ＩＳＫＮＶ ＰＣＲ检测方法对健康和ＩＳＫＮＶ感染后存活的鳜组织进行了检测，结果显示，健康鳜为阴性，而ＩＳＫＮＶ感染后存活的鱼，头肾、后肾、脾脏和部分鱼的心脏为阳性。ＩＳＷ在鳜体内形成潜伏感染。
The infectious spleen and kidney disease virus (ISKNV), concanavallin A (ConA) and lipopolysaccharide (LPS) were studied by WTT color reaction in vitro and in vivo for 30 days after ISKNV infection The effects of 60d 鳜 of head kidney lymphocyte transformation. The results showed that ConA and LPS could promote the conversion of healthy grade nephritic lymphocytes, but had no effect on trophoblastic lymphocytes that survived 30d or 60d after infection. Purified ISKNV did not promote lymphocyte transformation of healthy tau lymphocytes But inhibited the action of ConA. Purified ISKNV could promote the transformation after 30d or 60d of tumefacient renal lymphocytes survived infection. Infected goblet kidneys have immunoreactive T cells for ISKNV. In addition, the established ISKNV PCR assay was used to test the health and survival of gall bladder tissue after ISKNV infection. The results showed that the healthy goblet cells were negative, while the surviving fish, head kidney, kidney, spleen and part of fish survived ISKNV infection The heart is positive. ISW in the body form a latent infection.