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授粉后２５－３０天的花生幼胚，去子叶后，在ＭＳ２（ＭＳ＋ＢＡ１．０ｍｇ／Ｌ＋ＣＨ３００ｍｇ／Ｌ＋ＰＶＰＰ０．３％＋椰计５０ｍｌ／Ｌ＋蔗糖４％＋琼脂０．８％）培养基中，置３００ｌｕｘ弱光和２０℃±１℃条件下，培养２１－２８天，能有效地产生丛生芽；在ＭＳ２中诱导培养后的去子叶幼胚的生长点切去，在改良无激素培养基（ＭＳ１）中培养１４天，转入ＭＳ３（ＭＳ＋ＢＡ６．０ｍｇ／Ｌ＋ＮＡＡ０．４ｍｌ／Ｌ＋Ｄ－生物素０．１ｍｇ／Ｌ＋ＣＨ３００ｍｇ／Ｌ＋酵母浸出物２００ｍｇ／Ｌ＋椰计５０ｍ／Ｌ＋ＰＶＰＰ０．３％＋蔗糖２．５％＋琼脂０．８％）培养基，置２５℃±１℃，３５００ｌｕｘ光照条件下培养２１－２８天，８０％的外植体分化出丛生芽，继而长成无根带叶小苗．．平均每外植体产生８．１个丛生芽，最多可达４０个．诱根后小苗移植田间８０％植株成活并开花结实。品种（系）间差异不显著。
The peanut embryos 25-30 days after pollination were cotyledon, and after 300ml of culture in MS2 (MS + BA1.0mg / L + CH300mg / L + PVPP0.3% + coco 50ml / L + sucrose 4% + agar 0.8% Under low light and 20 ℃ ± 1 ℃, cultured 21-28 days, can effectively produce clusters of buds; induced MS2 induced cotyledon immature embryo growth point cut, in the modified hormone-free medium (MS1) (MS + BA6.0mg / L + NAA0.4ml / L + D-biotin 0.1mg / L + CH300mg / L + Yeast Extract200mg / L + Coconut 50m / L + PVPP0.3% + Sucrose 2.5% + agar 0.8%) medium at 25 ℃ ± 1 ℃, cultured under 3500 lux for 21-28 days. 80% of explants differentiated into clusters of buds and then grew into rootless leaflets. . An average of 8.1 clustered shoots per explant are generated, up to 40. After the induction of seedling transplant 80% of the plants in the field survive and flowering and fruiting. Varieties (lines) were not significantly different.