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用抗M CSF受体的单克隆抗体和兔多克隆抗体及ABC复合物 ,建立了检测M CSF可溶性受体 (sM CSFR的双抗体夹心ABC ELISA。本法灵敏度高 (0 1ng/ml) ,特异性强 ,重复性好 ,准确性高 (CV <10 % ) ,可检出肿瘤患者及部分正常人血清中可溶性sM CSFR的含量。本法的建立也为探讨sM CSFR的生物学功能及临床意义提供方法学基础。
A dual antibody sandwich ABC ELISA for the detection of M CSF soluble receptor (sM CSFR) was established using a monoclonal antibody against M CSF receptor and a rabbit polyclonal antibody and ABC complex. The method has high sensitivity (0 1 ng / ml), specificity The detection of soluble sM CSFR in the serum of patients with cancer and some normal persons can be detected by this method.The establishment of this method is also to explore the biological function and clinical significance of sM CSFR Provide a methodological foundation.