结核杆菌全菌裂解物和全脂质抗原对人外周血单个核细胞的作用

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目的观察结核杆菌全菌裂解物和全脂质抗原对人外周血单个核细胞(PBMC)活化和增殖的作用。方法健康成人PBMC(1×106/ml)分别加结核杆菌全菌裂解物(10μg/ml)或全脂质(10μg/ml)进行刺激,同时给予重组人白介素-2(rhIL-2,50 U/ml)维持细胞增殖,另外设仅加rhIL-2的对照组,培养至第6、9、12天,收集培养扩增细胞,流式细胞术检测T细胞以及不同亚群的增殖情况和IFN-γ的产生。结果 2组抗原均能刺激PBMC中T细胞的增殖和活化,第9天时反应最为明显,与对照组相比,全菌裂解物组CD8+T细胞比例降低而CD4-CD8-T细胞比例明显增高;全脂质组CD4+T细胞以及CD4+CD8+T细胞比例增高,差异有统计学意义(P<0.05)。培养至第12天,对分泌IFN-γ的细胞亚群分析,全菌裂解物组与全脂质CD8+T细胞所占比例均高于其相应的rhIL-2对照组(P<0.05);全菌裂解物组CD8-T细胞所占比例低于其相应对照组(P<0.05),而全脂质组高于其相应对照组(P<0.05)。结论结核杆菌全脂质抗原与全菌裂解物一样,能激活特异性免疫反应,具有潜在的疫苗组分可能性。 Objective To observe the effect of Mycobacterium tuberculosis lysate and whole lipid antigen on the activation and proliferation of human peripheral blood mononuclear cells (PBMCs). Methods PBMC of healthy adults (1 × 106 / ml) were stimulated with whole bacteria lysate of Mycobacterium tuberculosis (10μg / ml) or whole lipids (10μg / ml) / ml) to maintain cell proliferation. In addition, only rhIL-2-added control group was cultured to the 6th, 9th and 12th days, and the cultured expanded cells were collected. The proliferation of T cells and different subsets were detected by flow cytometry and IFN -γ production. Results Both groups of antigens could stimulate the proliferation and activation of T cells in PBMC, and the most obvious reaction was on the 9th day. Compared with the control group, the proportion of CD8 + T cells in the whole bacterial lysate group decreased and the proportion of CD4-CD8-T cells was significantly increased ; The proportion of CD4 + T cells and CD4 + CD8 + T cells in the whole lipid group increased, the difference was statistically significant (P <0.05). On the 12th day, the proportion of whole cell lysate group and whole lipids CD8 + T cells in IFN-γ-secreting cell subgroup was higher than that in rhIL-2 control group (P <0.05). The proportion of CD8-T cells in the whole lysate group was lower than that of the corresponding control group (P <0.05), while that in the whole lipid group was higher than that of the corresponding control group (P <0.05). Conclusion Mycobacterium tuberculosis whole lipids, like the whole bacterial lysate, activate the specific immune response and have the potential of vaccine components.
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