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锥形摇瓶是实验室广泛使用的培养微生物的器皿,这是因为其结构简单、操作方便,且在同一摇床上进行多种并行试验以满足菌种筛选、培养基成分优化、培养过程的周期和需氧程度的定性推测等要求。但摇瓶在结构上和供氧与气液传递的方法方式上与实验室或生产上用的生物反应器有很大的不同,且一般不能在培养过程中取样和补料,限制了它的应用并且难以将摇瓶的研究结果转移到生物反应器中或用于发酵罐的放大。为此,我们研制了两种可在线检测某些能反映微生物过程中物质传递和代谢的特制摇瓶,以期能克服一般摇瓶不足之处。其中Ⅰ型摇瓶能检测培养过程中的溶氧、摄氧率、氧传递系数,Ⅱ型摇瓶除了能检测上述3个参数外,还能检测CO2释放率和呼吸商,两者都能进行取样和补料。文中还介绍了利用特制摇瓶测得的摇瓶口过滤介质的氧通透率和对4种具体过程,即啤酒酵母的培养、固定化酵母将AR转化为ATP、rbFGF大肠埃希氏菌的培养和面包酵母将乙酰乙酸乙酯转化为[S]-3羟基丁酸乙酯的摇瓶和小生物反应器的对照试验。试验的结果初步认为:在氧敏感的纯培养中,OUR或DO是关键参数;在厌气过程中CER是关键参数;在次级代谢产物的发酵、微生物转化及某些重组菌的培养中,OUR和CER或R?
Conical flask is widely used in laboratory laboratory culture of microorganisms, because of its simple structure, easy to operate, and in the same shaker for a variety of parallel experiments to meet the bacteria screening, optimization of the composition of the medium, the incubation period And aerobic level of qualitative speculation and other requirements. However, shake flasks are very different in structure and in the manner of oxygen and gas-liquid transfer from laboratory or production bioreactors and generally can not be sampled and fed during the culture, limiting their Applied and difficult to transfer shake flask research results into bioreactors or for amplification of fermenters. To this end, we have developed two special on-line detection of certain shake flask can reflect the material transfer and metabolism of microorganisms in the process, in order to overcome the general bottle shake deficiencies. Type I shake flask can detect dissolved oxygen, oxygen uptake rate and oxygen transfer coefficient during culturing. Type II shake flask can detect CO2 release rate and respiratory quotient in addition to the above three parameters, both of which can be performed Sampling and feeding. The article also introduced the use of special shaker bottle shake measured bottleneck oxygen permeability of the filter medium and the four specific processes, namely, the cultivation of beer yeast immobilized yeast AR into ATP, rbFGF Escherichia coli Culture and Baker Yeast A control experiment for shake flask and small bioreactor that converts ethyl acetoacetate to ethyl [S] -3 hydroxybutyrate. The results of the experiment preliminarily suggest that OUR or DO is the key parameter in oxygen-sensitive pure culture; CER is the key parameter in anaerobic process; in the fermentation of secondary metabolites, microbial transformation and the culture of some recombinant bacteria, OUR and CER or R?