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研究白蛋白结合型紫杉醇(Abraxane~?)、胶束化紫杉醇(Genexol~?-PM)和紫杉醇注射液(Taxol~?)在大鼠血浆中游离药物浓度的差异,并考察冻-融处理对其中游离紫杉醇测定的影响。雄性SD大鼠静脉注射紫杉醇制剂,采集给药后2和4 h血浆样品,经3次-70℃冻-融,用快速平衡透析(RED)法处理。以多西他赛为内标,建立了液相色谱-串联质谱法测定血浆游离紫杉醇浓度。结果表明,白蛋白结合型紫杉醇和胶束化紫杉醇在大鼠血浆中游离药物浓度差异不显著(P>0.05),但明显高于紫杉醇注射液的游离药物浓度。血浆样品冻-融3次后不影响游离药物浓度的测定(P>0.05)。
To study the difference of free drug concentration in rat plasma between Abraxane ~ (?), Genexol ~ (-PM) and Taxol ~ (?), And investigate the effects of freeze-thaw treatment Among the effects of free paclitaxel assay. Male Sprague-Dawley rats were injected intravenously with paclitaxel. Plasma samples were collected at 2 and 4 h after administration. The plasma samples were frozen-thawed three times at -70 ° C and treated with rapid equilibrium dialysis (RED). To docetaxel as internal standard, established a liquid chromatography - tandem mass spectrometry determination of plasma free paclitaxel concentration. The results showed that albumin-bound paclitaxel and paclitaxel paclitaxel in plasma concentrations of free drugs was no significant difference (P> 0.05), but significantly higher than the free drug concentration of paclitaxel injection. Plasma samples frozen - melt three times without affecting the determination of free drug concentration (P> 0.05).