方便高效的保存方法是开展动物肠道微生物研究的重要前提,用T-RFLP(末端限制性片段长度多态性)结合16S rDNA分析评估了野外采样常用的DETs(20%DMSO,0.25 M sodium-EDTA,100 mM Tris,pH 7.5,and NaCl to saturation)缓冲液对不同保存时间(24h,7天,30天,90天)粪便样品肠道微生物DNA的保存效果。结果表明,DETs保存在30天保存时间内微生物群落结构无显著变化,95.6%的T-RFs仍然存在,能效好的保护肠道微生物DNA。 A convenient and efficient method of preservation is an important prerequisite for gut microbiota in animals. DETs commonly used in field sampling (20% DMSO, 0.25 M sodium- EDTA, 100 mM Tris, pH 7.5, and NaCl to saturation) buffer against intestinal microflora in stool samples at different storage times (24h, 7d, 30d, 90d). The results showed that there was no significant change in the structure of microbial communities after DETs were stored for 30 days, and 95.6% of T-RFs still existed to protect the intestinal microbial DNA.