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本工作以玻璃微电极记录脊髓腰段（Ｌ２－３）背角神经元电活动，观察血管紧张素Ⅱ（ＡⅡ）对其伤害性诱发放电的影响，并探讨ＡⅡ与吗啡抑制效应的相互关系。结果表明，ＡⅡ５０－５００ｎｇ脊髓表面微量滴注对背角神经元伤害性诱发放电主要为抑制效应，而ＡⅡ２μｇ为易化效应；注射吗啡（５ｍｇ／ｋｇ，ｉｐ）后１０ｍｈ；ＡⅡ２５０ｎｇ脊髓表面微量滴注不能对抗吗啡的抑制作用，而ＡⅡ２一４μｇ则能部分或完全抵消吗啡对背角神经元伤害性诱发放电的抑制作用。本工作提示，ＡⅡ可调制脊髓背角神经元对外周传入的伤害性反应；较大剂量ＡⅡ可对抗吗啡对伤害性诱发放电的抑制作用。
In this work, the electrical activity of neurons in the dorsal horn of spinal cord (L2-3) was recorded by glass microelectrode. The effect of angiotensin Ⅱ (AⅡ) on the nociceptive firing was observed and the relationship between AⅡ and morphine inhibition was also investigated. The results showed that microdial injection of AⅡ50-500ng on the spinal dorsal horn primarily inhibited the nociceptive discharge of the dorsal horn neurons, whereas AⅡ2μg was a facilitation effect; microinjection of 250ng into the spinal cord of AⅡ 250ng after injection of morphine (5mg / kg, ip) Can not antagonize the inhibition of morphine, and A Ⅱ 2 a 4μg can partially or completely offset by morphine on the dorsal horn neuronal injury induced discharge inhibition. This work suggests that AⅡ can modulate the spinal cord dorsal horn neurons into the peripheral nociceptive response; larger doses of A Ⅱ against morphine on the nociceptive discharge induced inhibition.