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以鲜食蚕豆(Vicia faba L.)品种“通蚕鲜6号”为试材,采用1/2 Hoagland营养液栽培,研究不同浓度NaCl(0、50、100、150mmol·L~(-1))处理对鲜食蚕豆幼苗生长、光合特性和抗氧化酶等生理代谢的影响,为盐碱地区蚕豆的种植提供参考依据。结果表明:盐处理时,蚕豆生长呈现低浓度(50mmol·L~(-1))促进,中高浓度(≥100mmol·L~(-1))抑制,尤其是高浓度处理组植株干质量比对照组下降了64.3%,并且在高浓度时蚕豆净光合速率、蒸腾速率、气孔导度和胞间CO_2浓度也均呈降低趋势,分别下降了19.7%、56.3%、74.0%和61.2%;但是叶绿素a、叶绿素b和叶绿素(a+b)的含量在3种盐浓度处理时均出现降低趋势;在低中浓度处理时,超氧化物歧化酶(SOD)活性升高,高浓度处理时,胁迫程度加深,酶活性大幅度下降;过氧化物酶(POD)随着盐浓度升高和时间的延长均呈现升高趋势;过氧化氢酶(CAT)在低中浓度时有上升现象,低浓度上升幅度更大,在高浓度时呈先上升后下降的趋势;3个处理组的MDA含量均随盐浓度升高呈现增长趋势。
Using 1/2 Hoagland nutrient solution, Vicia faba L. cultivar “Tongmuxian 6” was used to study the effects of different concentrations of NaCl (0, 50, 100, 150 mmol·L ~ (- 1)) on the growth, photosynthetic characteristics and antioxidase activities of fresh broad bean (Vicia faba L.) seedlings in order to provide reference for the cultivation of Vicia faba in saline-alkali soil. The results showed that: a salt treatment, exhibit a low concentration of broad bean (50mmol·L ~ (-1)) to promote, high concentrations (≥100mmol·L ~ (-1)) inhibition, especially for high total dry mass concentrations than the control group group decreased by 64.3%, and at high concentrations CO_2 concentration between Vicia net photosynthetic rate, transpiration rate, stomatal conductance and cell also decreasing trend, decreased by 19.7%, 56.3%, 74.0% and 61.2%; however chlorophyll a, chlorophyll b, and chlorophyll content (a + b) reduce the tendency of the three kinds are present at the concentration process; when treated at low concentration, superoxide dismutase (SOD) activity increased, high concentrations, Stress The level of peroxidase (POD) increased with the increase of salt concentration and time; the increase of catalase (CAT) was observed at low and medium concentrations; while at low concentration The content of MDA in the three treatments increased with the increasing of salt concentration.