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为探讨佛波酯对胃癌细胞肿瘤坏死因子（ＴＮＦ）受体的调变机制。以标记的肿瘤坏死因子突变体为配体，用放射配体结合分析法和ＭＴＴ比色法研究了佛波酯对ＴＮＦ受体的调节以及对肿瘤坏死因子突变体（ＴＮＦ－ｍ）细胞毒效应的影响。结果表明：佛波酯可导致胃癌细胞（ＳＧＣ７９０１）表面ＴＮＦ受体数显著减少（由０．５６×１０－１１ｎｍｏｌ／细胞，下降到０．２５×１０－１１ｎｍｏｌ／细胞，Ｐ＜０．０１），而对受体的亲和力无影响；佛波酯不影响ＴＮＦ－ｍ的内化率、降解率和解离率，佛波酯也不改变胞浆ＴＮＦ受体数目；佛波酯处理后的ＳＧＣ７９０１细胞对肿瘤坏死因子突变体（ＴＮＦ－ｍ）的耐受性显著增强。去除佛波酯后１２ｈ，ＴＮＦ受体可恢复８０％，其恢复率与胰酶处理组相似。从而提示：佛波酯可能通过清除膜ＴＮＦ受体而使ＴＮＦ受体下调并抑制ＴＮＦ－ｍ的细胞毒效应
To investigate the modulation mechanism of phorbol ester on tumor necrosis factor (TNF) receptor in gastric cancer cells. The tumor tumour necrosis factor mutants were used as ligands. The modulation of phorbol esters on TNF receptors and the cytotoxicity of tumor necrosis factor mutant (TNF-m) were studied by radioligand binding assay and MTT colorimetry. Impact. The results showed that phorbol ester can significantly reduce the number of TNF receptors on gastric cancer cells (SGC7901) (from 0.56×10-11 nmol/cell to 0.25×10-11 nmol/cell, P<0.01). No influence on receptor affinity; Phorbol ester does not affect the internalization rate, degradation rate, and dissociation rate of TNF-m, and phorbol ester does not change the number of cytoplasmic TNF receptors; SGB7901 cells treated with phorbol ester Tolerance to tumor necrosis factor mutants (TNF-m) was significantly enhanced. After 12 hours of phorbol ester removal, TNF receptors recovered 80%, and the recovery rate was similar to that of the pancreatic enzyme treatment group. This suggests that phorbol esters may downregulate TNF receptors and suppress the cytotoxic effects of TNF-m by eliminating membrane TNF receptors.