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目的研究L-plastin启动子单核苷酸多态性(SNP)与前列腺癌的关系。方法采用Tm-shifting(等位基因特异性扩增结合融解温度曲线分析法)分析方法,对82例前列腺癌人群和94例对照人群的L-plastin启动子SNP位点的基因型进行检测。结果 L-plastin启动子TT、CT、CC基因型及等位基因T、C在汉族前列腺癌患者及对照者中的分布频率差异无显著性(P>0.05);在前列腺癌组内不同病理分级中,中、高分化组和低分化组中TT、CT、CC基因型分布频率差异无显著性(P>0.05);在前列腺癌组内不同临床分期中,与TT纯合子相比,CC纯合子前列腺癌Ⅳ期的风险增加至5.0倍(P<0.05)。结论 L-plastin启动子单核苷酸多态性在中国汉族人群中与前列腺癌无相关,但该启动子多态性可能在预测前列腺癌进展中有一定作用。
Objective To investigate the relationship between L-plastin promoter single nucleotide polymorphism (SNP) and prostate cancer. Methods The genotypes of L-plastin promoter SNP in 82 prostate cancer patients and 94 control subjects were detected by Tm-shifting assay. Results There was no significant difference in the distribution frequencies of TT, CT, CC genotypes and alleles T, C of L-plastin promoter in Han patients with prostate cancer and controls (P> 0.05) There was no significant difference in the frequencies of TT, CT and CC genotypes between the moderately, moderately differentiated and poorly differentiated groups (P> 0.05). In the different clinical stages of the prostate cancer group, compared with the TT homozygotes, CC The risk of Zygotic Prostate Stage IV increased 5.0-fold (P <0.05). Conclusion L-plastin promoter SNPs have no correlation with prostate cancer in Chinese Han population, but the promoter polymorphism may play a role in predicting the progression of prostate cancer.