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[Objective] To find the optimal culture conditions of Se-enriched Ganoderma lucidum (Leyss.ex Fr.) Karst. mycelium. [Method] The effects of carbon source, nitrogen source and fermentation conditions on the dry weight, total selenium and organic selenium content of Se-enriched G. lucidum mycelium were studied by using single factor test and orthogonal test. [Result] Under conditions of 0.01% sodium selenium content, glucose content 4%, potato extracting 20%, peptone content 1%, KH2PO4 0.3%, MgSO4 0.15%, pH=7.0, inoculation amount 10%, culture temperature 28 ℃, and shaking speed 180 r/min, the dry weight of Se-enriched G. lucidum mycelium was 15.28 mg/ml, and the organic selenium content was 4 115.36 μg/g. [Conclusion] The optimal culture conditions of Se-enriched G. lucidum were determined, which could provide basis for development and utilization of Se-enriched G. lucidum.
[Objective] To find the optimal culture conditions of Se-enriched Ganoderma lucidum (Leyss. Ex Fr.) Karst. Mycelium. [Method] The effects of carbon source, nitrogen source and fermentation conditions on the dry weight, total selenium and organic selenium content of Se-enriched G. lucidum mycelium were studied by using single factor test and orthogonal test. [Result] Under conditions of 0.01% sodium selenium content, glucose content 4%, potato extract 20%, peptone content 1%, KH2PO4 0.3% , MgSO4 0.15%, pH 7.0, inoculation amount 10%, culture temperature 28 ° C, and shaking speed 180 r / min, the dry weight of Se-enriched G. lucidum mycelium was 15.28 mg / ml, and the organic selenium content was 4 115.36 μg / g. [Conclusion] The optimal culture conditions of Se-enriched G. lucidum were determined, which could provide basis for development and utilization of Se-enriched G. lucidum.