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目的:研究脱落酸(ABA)及其生物合成抑制剂氟啶酮(fluridone),对丹参毛状根酚酸类成分和关键酶的影响。方法:继代培养18 d的丹参毛状根添加不同浓度的ABA及ABA与氟啶酮组合,处理1 d后测定关键酶PAL和TAT活性;处理6 d后测定不同处理的丹参毛状根中酚酸类物质的含量。结果:在一定浓度范围内,低浓度的ABA促进丹参毛状根的生长,高浓度的ABA抑制丹参毛状根的生长;ABA显著促进酚酸类物质的积累,对咖啡酸的诱导表现出正相关,但是迷迭香酸和丹酚酸B的效果,表现出在低浓度效果较好,随浓度增大,诱导效果降低,直到ABA浓度至200μmol.L-1,含量开始上升;当ABA与氟啶酮组合处理时,氟啶酮抑制ABA对丹参毛状根酚酸类的积累,但是有差异;不同浓度的ABA会不同程度的提高PAL和TAT酶的活性,添加ABA生物合成抑制剂,酶活性诱导效果降低。结论:ABA能诱导丹参毛状根中酚酸类化合物积累,同时也能激活合成相关酶的活性;ABA生物合成抑制剂氟啶酮不同程度抑制ABA的诱导效果。
Objective: To study the effects of abscisic acid (ABA) and its biosynthesis inhibitor fluridone on the constituents and key enzymes of the hair root phenols in Salvia miltiorrhiza. METHODS: The hairy roots of Salvia miltiorrhiza were cultured for 18 days. Different concentrations of ABA and ABA were combined with fluridone to determine the activities of PAL and TAT. After 6 days of treatment, Phenolic acids content. Results: In a certain concentration range, low concentration of ABA promoted the growth of hairy root of Salvia miltiorrhiza, while high concentration of ABA inhibited the growth of hairy root of Salvia miltiorrhiza. ABA significantly promoted the accumulation of phenolic acids and showed a positive effect on caffeic acid However, the effect of rosmarinic acid and salvianolic acid B showed that the effect was better at low concentration. With the increase of concentration, the induction effect decreased until the content of ABA reached 200μmol.L-1, Fludizid inhibits ABA accumulation in the salvia miltiorrhiza hairpin phenolic acids, but different concentrations of ABA can increase PAL and TAT enzyme activity to some extent, add ABA biosynthesis inhibitors, Enzyme activity induced effect decreased. CONCLUSION: ABA can induce the accumulation of phenolic acids in the hairy roots of Salvia miltiorrhiza, but also activate the activity of synthetic enzymes. The ABA biosynthesis inhibitor, fluoridone, inhibits the induction of ABA to varying degrees.