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为了探明小麦高温抗条锈性的遗传机理,以小麦条锈菌流行小种对小麦品种小偃54和小偃81进行了苗期接种鉴定,并采用RT-PCR方法从小偃54中克隆了几丁质酶基因Chi1编码区全长cDNA序列Chi1xy,利用半定量PCR技术比较分析了常温(14±1℃)和高温(21±1℃)处理下条锈菌侵染的小偃54几丁质酶基因Chi1xy的表达谱。结果表明,小偃54和小偃81均具有明显的高温抗条锈性。常温处理下,Chi1xy的表达没有明显变化,始终维持在较低水平。而高温处理下,Chi1xy的表达在处理后的前72 h变化不明显,96 h开始升高,并在144 h达到表达高峰,直到192 h仍维持较高的表达水平。两种温度处理下Chi1xy的表达谱差异显著,说明小偃54对条锈病的高温抗条锈性可能与几丁质酶基因Chi1xy的表达水平相关。这是首次发现几丁质酶和高温抗条锈性有关。
In order to explore the genetic mechanism of high temperature resistance to stripe rust in wheat, wheat varieties Xiaoyan 54 and Xiaoyan 81 were identified inoculation at seedling stage with wheat stripe rust race and cloned from Xiaoyan 54 by RT-PCR The full-length cDNA sequence of chitinase gene Chi1 coding region Chi1xy was analyzed by semi-quantitative PCR. The expression profile of the Chi1xyzymes gene. The results showed that Xiaoyan 54 and Xiaoyan 81 all had obvious high temperature resistance to stripe rust. Under normal temperature, the expression of Chi1xy did not change significantly, and remained at a low level all the time. Under high temperature treatment, the expression of Chi1xy did not change significantly at 72 h after treatment and began to increase at 96 h, reaching the peak at 144 h, and remained high until 192 h. The expression profiles of Chi1xy under two temperature treatments showed significant differences, indicating that the high temperature resistance to stripe rust of Xiaoyan 54 might be related to the expression level of chitinase gene Chi1xy. This is the first time that chitinase has been found to be associated with high temperature resistance to stripe rust.