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目的:探讨糖毒清治疗糖尿病肾病肾功能不全的可能机制。方法:采用5/6肾切除合中等剂量的STZ腹腔注射方法建立糖尿病肾病肾功能不全模型,将造模成功的大鼠50只随机分为5组:模型组(生理盐水2mL/d灌胃)、糖毒清高剂量组[糖毒清6.75g/(kg·d)灌胃];糖毒清中剂量组[糖毒清4.5g/(kg·d)灌胃]、糖毒清低剂量组[糖毒清2.25g/(kg·d)灌胃]、尿毒清组[尿毒清2.25g/(kg·d)灌胃]。另外10只正常对照组大鼠予生理盐水2mL/d灌胃;治疗10周后,通过免疫组化的方法检测各组大鼠肾组织的VCAM-1表达情况。结果:模型组大鼠肾组织VCAM-1表达明显增多,而糖毒清组大鼠肾组织VCAM-1的表达明显减少。结论:糖毒清可以减少糖尿病肾病肾功能不全大鼠肾组织VCAM-1的过度表达,从而达到控制或延缓糖尿病肾病肾功能恶化的进程。
Objective: To investigate the possible mechanism of Tangduqing in the treatment of diabetic nephropathy with renal insufficiency. METHODS: The renal insufficiency model of diabetic nephropathy was established by 5/6 nephrectomy with moderate dose of STZ intraperitoneal injection. 50 rats with successful modeling were randomly divided into 5 groups: model group (normal saline 2 mL/d gavage) High-dose sugar-toxin-clearing group [Guanduqing 6.75g/(kg·d) gavage]; Tangduqing mid-dose group [sugar toxin 4.5g/(kg·d) gavage], low-dose Tangmuqing group [Sugar Toxic 2.25g/(kg·d) gavage], uremic urinary clear group [urinary toxin 2.25g/(kg·d) gavage]. The other 10 normal control rats were intragastricly administered with normal saline at 2 mL/d. After 10 weeks of treatment, the expression of VCAM-1 in the kidneys of each group was detected by immunohistochemistry. RESULTS: The expression of VCAM-1 in kidney tissue of the model group increased significantly, while the expression of VCAM-1 in kidney tissue of glycoside was significantly decreased. Conclusion: Tangduqing can reduce the overexpression of VCAM-1 in renal tissue of rats with diabetic nephropathy with renal insufficiency, so as to control or delay the progression of renal dysfunction in diabetic nephropathy.