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目的:研究半夏生物总碱对人肝癌细胞Bel-7402增殖的影响。方法:采用MTT比色法以及集落形成率来测定半夏生物碱对Bel-7402生长抑制作用。结果:半夏生物碱400,200,100μg·ml~(-1)对肝癌细胞Bel-7402有明显抑制作用(P<0.05或0.01),随着半夏生物碱浓度的增加和共同培养时间的延长,抑制作用逐渐增强。结论:半夏生物碱对人肝癌细胞Bel-7402生长有一定的抑制作用。
OBJECTIVE: To study the effect of Pinellia Biogen on the proliferation of human hepatoma Bel-7402 cells. Methods: MTT colorimetric assay and colony formation rate were used to determine the inhibition of Bel-7402 alkaloids on Bel-7402 growth. RESULTS: The concentration of 400, 200, 100 μg·ml -1 of Pinellia alkaloids significantly inhibited Bel-7402 cells (P<0.05 or 0.01). With the increase of alkaloid concentration in Pinellia ternata and the prolongation of co-cultivation time, the inhibitory effect was inhibited. The role gradually increased. Conclusion: Pinellia alkaloids can inhibit the growth of human hepatoma Bel-7402 cells.