从中国浙江省舟山渔场油污染的海水和海洋沉积物中分离筛选产生物表面活性剂的柴油降解菌株。经富集培养、形态观察、测定单菌噬油斑、柴油降解率大小初筛到3株柴油降解菌。然后对初筛到的3株菌进行液滴坍塌实验、发酵液的表面张力、排油圈和乳化稳定性的大小测定进一步复筛,最终筛选出1株产生物表面活性剂的柴油降解菌,经18s rRNA鉴定为海洋解脂耶罗威亚酵母(Yarrowia lipolytica)。其柴油降解率为80%,发酵液液体表面张力可从73.4 m N/m降至23.56 m N/m,乳化效率E24为60%。通过薄层色谱、傅里叶变换红外光谱、GC/MS鉴定,其产生的表面活性剂是由C14、C15β-羟基脂肪酸组成的脂肽。 Diesel oil degrading strains producing surfactant were separated and identified from the oil-contaminated seawater and marine sediment of Zhoushan Fishing Ground in Zhejiang Province, China. After enrichment culture and morphological observation, three strains of diesel-degrading bacteria were initially screened for the determination of single-engine macrophage plaque and diesel degradation rate. Then three droplets of the primary screening were tested for droplet collapse, the surface tension of the fermentation broth, the size of the drainage circle and the emulsion stability were further sieved, and finally one diesel-degrading bacterium producing the surfactant was screened out. The 18s rRNA was identified as Yarrowia lipolytica. The diesel oil degradation rate was 80%, the liquid surface tension of the fermentation liquid could be reduced from 73.4 m N / m to 23.56 m N / m, and the emulsification efficiency E24 was 60%. By TLC, FTIR, GC / MS identification, the resulting surfactant is a lipopeptide consisting of C14, C15 beta-hydroxy fatty acids.