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作者在体外观察了恶性疟原虫FCR株(乙胺嘧啶敏感株,IC_(50)=3)和W_2株(乙胺嘧啶抗性株,IC_(50)=30000)对[(14)C)乙胺嘧啶的摄入、乙胺嘧啶的代谢以及二氢叶酸还原酶(DHFR)酶促反应的动力学特征。实验采用含8％高度同步化的14小时龄环状体原虫与20μM的[~(14)C]乙胺嘧啶共同孵育24小时,液体闪烁计数器测定[~(14)C]乙胺嘧啶的放射性,并推算疟原虫摄入乙胺嘧啶的量。结果,FCR株与W_2株摄入量无差异。对照组每10~9正常红细胞摄入乙胺嘧啶0.07nmol,感染疟原虫的红细胞摄入0.24nmol,另用放
The authors observed the inhibitory effect of FCR (pymetrozine sensitive strain, IC 50) and W_2 strain (pyrimethamine resistant strain, IC 50 = 30000) against [14 C] Amine pyrimidine uptake, pyrimethamine metabolism, and kinetic characteristics of dihydrofolate reductase (DHFR) enzymatic reactions. The experiment was conducted for 24 hours with 14-hour-old protozoal protozoa containing 8% height synchronization and 20 μM [~ (14) C] pyrimethamine. The radioactivity of [~ (14) C] pyrimethamine was determined by a liquid scintillation counter , And calculate the amount of pyrimethamine intake of pyrimethamine. Results, FCR strain and W_2 strain no difference in intake. In the control group, every 10 to 9 normal erythrocytes uptake pyrimethamine 0.07nmol, erythrocytes infected with malaria parasites 0.24nmol, the other with