在以电化学方法确认L半胱氨酸对铜锌超氧化物歧化酶(copper,zincsuperoxidedismutase,SOD)在金丝电极上的电子迁移过程起促进作用的基础上,本文论述了将拉曼光谱电化学方法应用于猪红细胞SOD在经L半胱氨酸修饰的金丝电极上的电化学行为的现场拉曼光谱研究。研究结果从分子水平上提供了有关L半胱氨酸修饰在金电极上的电化学过程中,以及SOD在L半胱氨酸修饰的金电极上发生电子迁移过程中,存在于SOD、L半胱氨酸与金电极之间的相互成键作用情况随电化学过程进行而发生变化的微观结构信息,并进行了必要的分析和探讨。 Based on the electrochemical evidence that L-cysteine ​​can promote the electron transport of copper and zinc superoxide dismutase (SOD) on the gold electrode, this paper discussed the effect of Raman spectroscopy In situ Raman spectroscopic study of the electrochemical behavior of pig erythrocyte SOD on gold nanoparticles modified with L - cysteine. The findings provide molecular evidence that the electrochemical processes involved in the modification of L-cysteine ​​at gold electrodes and the migration of SOD at L-cysteine-modified gold electrodes occur during electron transport in the SOD, L-half Cystine and gold electrode bonding between the role of the electrochemical process with changes in the microstructure of the information, and the necessary analysis and discussion.