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利用甲基磺酸乙酯(EMS)诱变粳稻品种兰胜获得了一个能稳定遗传的矮化突变体ddu1。GA3点滴诱导水稻第2叶叶鞘伸长和α-淀粉酶诱导反应表明,ddu1并非为GA的缺陷型和信号传导阻碍矮化。将该突变体与籼稻浙辐802、明恢63和粳稻品种日本晴进行正反交配组,遗传分析表明该突变体受隐性单基因控制,而等位性检测表明ddu1与d1、d18、eui1和eui2均不等位。通过SSR和STS分子标记对F2代分离群体进行遗传定位,将该基因定位于第7染色体SSR标记RM427附近,随后又发展了多对有多态性的SSR和STS分子标记,最终将该基因定位于STS标记R5309和R3742之间,遗传距离分别为0.4和2.0 c M。
A dwarf mutant ddu1, which can be stably inherited, was obtained from a variety of japonica rice, LanSheng, using ethyl methanesulfonate (EMS). GA3 drop induced leaf elongation and α-amylase induction in the second leaf of rice showed that ddu1 was not deficient for GA and signal transduction impeded dwarfing. The mutant was crossed with the indica rice Xiefu 802, Minghui 63 and the Japonica variety Nipponbare by genetic analysis, and the genetic analysis indicated that the mutant was controlled by the recessive single gene. The allelic test showed that the combination of ddu1 and d1, d18, eui1 and eui2 are not equal. The SSR and STS molecular markers of F2 segregating population were used for genetic mapping. The gene was located in the vicinity of RM427 SSR marker on chromosome 7, followed by the development of multiple pairs of polymorphic SSR and STS molecular markers that ultimately mapped the gene Between the STS markers R5309 and R3742, the genetic distances were 0.4 and 2.0 cM, respectively.