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目的为筛选检验血、尿中未知安眠镇静药和三环抗忧郁药建立一种简便、快速的自动固相萃取方法。方法采用美国Zymark公司的自动固相萃取仪和Waters公司的OasisHLB固相萃取柱,同步提取血、尿中的25种常见安眠镇静药(包括巴比妥类、苯二氮卓类、吩噻嗪类药物等)和三环抗忧郁药。结果尿中药物提取率大部分达到80%以上;血中药物提取率在75%以上。结论该方法具有提取过程自动化,操作简便快捷,提取回收率高、重现性好、提取物干净等特点,可用于实际案件中血、尿中未知安眠镇静药和三环抗忧郁药的提取检验。
OBJECTIVE To establish a simple and rapid automatic SPE method for the screening of unknown sedatives and tricyclic antidepressants in blood and urine. Methods A total of 25 common sedative and anti-sedative drugs (including barbiturates, benzodiazepines, phenothiazines and phenothiazines) in blood and urine were extracted simultaneously by Zymark’s automated solid phase extraction (SPE) and Waters OasisHLB solid phase extraction Drugs, etc.) and tricyclic antidepressants. Results Most of the urine drug extraction rate reached more than 80%; blood drug extraction rate of 75%. Conclusion The method has the characteristics of automatic extraction process, simple and quick operation, high extraction recovery rate, good reproducibility and clean extract. It can be used in the extraction test of unknown sedative and tricyclic antidepressants in blood and urine in real cases .